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KMID : 0881720170320050404
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 5 p.404 ~ p.410
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Establishment of Analytical Method for Propylene Glycol Alginate in Food Products by Size-exclusion Chromatography
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Jeong Eun-Jeong
Choi Yoo-Jeong
Lee Gun-Young
Yun Sang-Soon
Lim Ho-Soo
Kim Yong-Suk
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Abstract
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An analytical method for determination of propylene glycol alginate (PGA) in food products was developed by HPLC-size-exclusion chromatography. The GF-7M HQ column and LT-ELSD detector were determined by considering the instrumental analysis conditions for PGA analysis. The pretreatment method for the analysis of PGA was suitable for 3 hr extraction at 20°C and 150 rpm according to the extraction temperature. Linearity (R2) for the analysis of PGA was 0.9873 at calibration curve range of 300, 500, 700, 1,000, and 1,500 mg/kg (5 points). The limit of detection and limit of quantification of PGA on HPLC system was 171.43 and 519.50 mg/kg, respectively. The accuracy and coefficient of variation obtained by size-exclusion chromatography were 86.1~110.4% and 4.1~13.5%, respectively. By applying the HPLC-size-exclusion chromatography system, it was possible to analyze the contents of PGA in 134 different types of food products.
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KEYWORD
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Propylene Glycol Alginate , Size-exclusion Chromatography , Food Additives , Analytical Method , Method Validation
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